Fig. 6

Putative LSCs detected in AML1-ETO pair (s220). (A) UMAP of Dx and Re cells for AML1-ETO patient s220, colored by timepoint (top) and cell cluster (bottom). (B) Heatmap depicting the top 5 marker genes per cluster. Color represents row normalized expression values. (C) LSC17 scores per cluster. * p < 0.05, ** p < 0.01, *** p < 0.001, Student’s t-test. (D) top: Barplots depicting the relative cell abundance per cell cycle phase (inferred from marker gene expression) for each cell cluster. Arrow: cells in cluster 4 and 5 predominantly reside in the G1 phase. Bottom: UMAP colored by cell cycle phase. (E) Pseudo-time trajectory colored by cell cluster (F) Heatmap depicting representative marker genes per cluster/inferred timepoint