Fig. 1
Conditional GLuc reporter mice. a Targeting strategy for Gt(ROSA)26Sortm2(CAG−GLuc) mice: insertion of a GLuc cDNA expression cassette controlled by the cytomegalovirus early enhancer/chicken beta actin (CAG) promoter and a loxP-flanked transcriptional stop cassette (LSL) into intron 1 of the Gt(ROSA)26Sor gene locus. b GLuc activity measured in organ lysates of mice with indicated genotypes (n = 3 biological replicates). c GLuc tissue activity. Each data point represents one tissue type (n = 11 tissues; P-values from Dunnett’s multiple comparisons test). d, e Level and temporal stability of GLuc activity in blood plasma of mice with indicated genotypes. d Time course, n = 3 mice per genotype. e Time average ± SD with data points representing individual time points (n = 24 time points; P-values from Dunnett’s multiple comparisons test