Fig. 5

Molecular characterization and GLuc-based monitoring of CRISPR-induced SCLC. a-c Histological analysis of AV-PR.CC9 induced a primary lung tumors and metastases to the b liver and c kidney. Shown are representative H&E and immunohistochemical stains for GLuc and NE lineage markers (Ascl1, Synaptophysin). d Temporal development of blood GLuc activity in individual mice following infection with indicated AVs (AV-C.CC9 n = 12; AV-PR.CC9 n = 8). Shaded area represents the GLuc background activity. e Trp53 and Rb1 mutation spectra of single AV-PR.CC9 tumors from 5 different mice. Top graph, shown is the frequency of wild-type and mutant reads. For mutant reads, all mutations with a frequency of > 5% are color-coded as deletions or insertions and labelled with the number of deleted or inserted base pairs. Less frequent mutations are summarized as ‘others’. Bottom graph, shown is the size distribution of indel mutations for each tumor. The frequency is each indel mutations is encoded in grayscale