Fig. 1
From: A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR
circZKSCAN1 is down-regulated in liver cancer tissues and is sensitive to sorafenib treatment. A Human adult normal liver datasets were analyses for differentially expressed circRNAs. The top 5 up-regulated circRNAs were at list. B Relative expression level of the top 5 up-regulated circRNAs in 30 pairs of HCC and normal tissues. C Relative expression level of the top 5 circRNAs in normal hepatocyte lines (THL-2) and HCC cell lines (SNU-387, SNU-398, HCC-LM3, Hep3B, Huh-7) by RT-qPCR analysis. D Schematic illustration exhibited the significantly different expressions circRNAs by overlapping GSE143233 and GSE140202. E The relative expression of circZKSCAN1, hsa_circ_0006886 and hsa_circ_0071106 in HCC cells with and without sorafenib treatment. F RT-qPCR analysis showed QKI-5 expression level in HCC-LM3 and Hep3B after different concentrations of sorafenib treatment. G RT-qPCR analysis showed expression levels of circZKSCAN1 and ZKSCAN1 in overexpressed QKI-5 HCC-LM3 and Hep3B cells. H RIP analysis showed expression levels of circZKSCAN1 in overexpressed QKI-5 HCC-LM3 cells. H Analysis of apoptosis in HCC-LM3 and Hep3B with or without overexpressed circZKSCAN1 transfection by flow cytometry assays. T test was performed for significant analysis, significant differences are indicated with * for p < 0.05, ** for p < 0.01 and *** for p < 0.001