Fig. 2
From: A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR
circZKSCAN1 encodes a 206aa peptides. A Vectors schematic diagram showed two IRSE sequences in circZKSCAN1 were cloned into Psi-check2 vector. B The relative luciferase activity of Luc/Rluc was used to tested circZKSCAN1 translation ability in HCC-LM3 cells, Encephalomyocarditis Virus (EMCV) IRES was used as a positive control, empty vector was served as a negative control. C Polysome fractionation and RT-PCR analysis after lysate of HCC-LM3 cells, GAPDH serve as a positive control. D Vectors schematic diagram showed that His/HA-labeled circZKSCAN1-mut, circZKSCAN1, Vector and circZKSaa plasmid. E Upper panel, RT-qPCR results of circZKSCAN1 on vector (circZKSCAN1-mut, circZKSCAN1, Vector and circZKSaa) transfected 293 T cells. Lower panel showed Western bolt assay of circZKSaa expression level on vector (circZKSCAN1-mut, circZKSCAN1, Vector and circZKSaa) transfected 293 T cells. F Western blot analyses showed circZKSaa expression of HCC-LM3, Hep3B and THL-2. G After Co-IP by HA antibody and separation by SDS-PAGE, the protein sample which stained by silver staining was analyzed by mass spectrometry. Data are presented as the mean ± SEM of three independent experiments