Fig. 3
From: A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR
circZKSaa has diagnostic value in HCC. A The circZKSaa content was tested via Western blot in 12 pairs of HCC and normal tissues. β-actin was used to indicate the amount of loading proteins. B Diagnostic value of circZKSaa in 29 pairs of HCC and normal tissues by ROC curve. C After transfected circZKSaa plasmid in HCC-LM3 and 293 T cells, the circZKSaa expression level in cells supernatant. D The RNA-Seq databases of HCC patient’s serum databases was analyzed by Linux’s CIRIquant, find_circ and other softwares. E The location of circZKSaa was determined by Nucleo-cytoplasmic separation, GAPDH was used as the positive control of cytoplasmic protein, while PCNA was used as the positive control of nuclear protein. F Western blot assay showed circZKSaa expression level in HCC-LM3 cells after treated sorafenib with different concentrations and different duration. G Analysis of apoptosis in HCC-LM3 and Hep3B with or without circZKSaa transfection by flow cytometry assays. Quantitative data from three independent experiments are presented as mean ± SEM (error bars). T test was performed for significant analysis, significant differences are indicated with * for p < 0.05, ** for p < 0.01 and *** for p < 0.001