Fig. 7
From: A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR
circZKSCaa promotes ubiquitination of mTOR. A RT-qPCR demonstrated mTOR and 5’UTR-mTOR expression level in circZKSCAN1-mut\circZKSCAN1\sh-scramble\sh-circZKSCAN1\Vector\circZKSaa transfected cells (HCC-LM3 and Hep3B). B Western blot detected half-life of mTOR by adding CHX for different times. The amount of mTOR protein level was calculated by ImageJ. C Western blot analyses mTOR protein degradation by MG-132 treatment. D Before treated with MG132, HCC-LM3 and Hep3B cells were transfected with HA-ubiquitin and circZKSaa-Flag. Whole cell lysates were IP with mTOR antibody followed by detection with ubiquitin antibody. GAPDH, Flag and mTOR serve as amount of input proteins. Quantitative data from three independent experiments are presented as mean ± SEM (error bars). T test was performed for significant analysis, significant differences are indicated with * for p < 0.05, ** for p < 0.01 and *** for p < 0.001