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Fig. 8 | Molecular Cancer

Fig. 8

From: A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR

Fig. 8

circZKSCaa degrades mTOR by interacting with FBXW7. A Co-IP assay by His antibody measured the interaction between mTOR, circZKSaa-His and FBXW7 in HCC-LM3 and Hep3B cells; Immunofluorescence staining of FBXW7-HA and circZKSaa-His in HCC-LM3 cells, Scale bars = 200 μm. B Western blot analyses mTOR ubiquitination level in FBXW7-overexpresion cells with or without down-regulated circZKSCAN1, both cells were pre-incubated with MG-132. C Western blot analyses mTOR ubiquitination level in circZKSaa-His overexpresion cells with or without down-regulated FBXW7, both cells were pre-incubated with MG-132. D FBXW7-HA, mTOR-Flag, circZKSaa-His vector plasmid was transfected into 293 T cells as indicated. Immunoprecipitation was performed with anti-Flag protein A/G magnetic beads and proteins from the immunoprecipitatte was subjected to Western blot for evaluation of FBXW7-HA and mTOR-Flag. E mTOR-Flag, mTOR-631/635-mut-Flag, circZKSaa-His vector plasmid was transfected into 293 T cells as indicated. Immunoprecipitation was performed with anti-Flag protein A/G magnetic beads and proteins from the immunoprecipitatte was subjected to Western blot for evaluation of circZKSaa-His and mTOR-Flag. F FBXW7-HA, mTOR-Flag, mTOR-631/635-mut-Flag, circZKSaa-His vector plasmid was transfected into 293 T cells as indicated. Immunoprecipitation was performed with anti-Flag protein A/G magnetic beads and proteins from the immunoprecipitatte was subjected to Western blot for evaluation of Ub and mTOR-Flag

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