Fig. 4

LH1 stabilizes SEPT2 using its hydroxylase function A Western blot showing the upregulation of SEPT2 by LH1 overexpression in SK-Hep1 and SNU449 cells. B Western blot showing the upregulation of SEPT2 by LH1 overexpression in PANC1 cells. C Western blot showing the downregulation of SEPT2 by LH1 knockdown in PLC/PRF/5 and SK-Hep1 cells. D Western blot showing the chloroquine treatment could reduce the degradation of SEPT2 protein. E&F Co-immunoprecipitation showing the binding of SEPT2 to SEPT3/6/7 in control and LH1-overexpressing SK-Hep1 (E) and SNU449 (F) cells. G Immunofluorescence showing the septin network in LH1-overexpressing SK-Hep1 and PANC1 cells. H Statistic result of hydroxylation level of SEPT2 protein in LH1 vector and overexpression group. I&J Confined migration of SNU449 (I) or SK-Hep1 (J) cells transfected with LH1 truncations. K Co-immunoprecipitation with 6 × His antibody showed that the truncated LH1 proteins could not bind with SEPT2. *, P < 0.05;**, P < 0.01;***, P < 0.001