Fig. 2

Higher CDR1as level indicated lymph node metastasis and worse overall survival of patients with cervical cancer. A To detect if CDR1as is a circular RNA, divergent and convergent primers were designed, agarose gel electrophoresis showed that CDR1as was amplified by divergent primer in cDNA but not in gDNA. GAPDH was taken as the negative control; B RNase R treatment assay was performed, and only CDR1as showed strong stability but not its linear origin; C Chromosome location and transcripts of CDR1as; D, E Nuclear-plasma extraction assay and FISH showed that CDR1as was mainly expressed in cytoplasm; F ISH was used to evaluate the expression level of CDR1as in cervical cancer tissue; G, H, I, J CDR1as level among cervical cancer tissue was analyzed by lymph node metastasis, tumor FIGO stage, tumor grade, and tumor size; *P < 0.05, **P < 0.01; ns, not significant; unpaired Student’s t test K, L K-M analysis of CDR1as in 111 cervical cancer tissue samples