Fig. 5
From: LncRNA-BC069792 suppresses tumor progression by targeting KCNQ4 in breast cancer
BC069792 upregulates the expression of KCNQ4 by binding to miR-658 and miR-4739. a Compared with the IgG control group, endogenous BC069792 and KCNQ4-3'UTR were specifically enriched by Ago2 antibody (BC069792: P = 0.017; FOS: P = 0.012; KCNQ4-3'UTR:P < 0.0001). b Through website prediction, database screening and literature query, three miRNAs were identified for further research. c After co-transfection of pmirGLO-BC069792 and mimics in MDA-MB-231 and MDA-MB-468 cell lines, miR-658 (MDA-MB-231: P = 0.021, MDA-MB-468: P = 0.037) and miR-658 -4739 (MDA-MB-231: P = 0.019; MDA-MB-468: P = 0.029) bound to pmirGLO-BC069792 and significantly inhibit luciferase activity. d After co-transfection of pmirGLO-KCNQ4 3'UTR and mimics, both miR-658 (MDA-MB- 231:P = 0.032, MDA-MB- 468:P = 0.017) and miR-4739(MDA-MB-231: P = 0.031; MDA-MB-468:P = 0.046) bound to pmirGLO-KCNQ4 3'UTR and reduced dual-luciferase activity. e There was no significant difference in luciferase activity between the experimental group and the control group after co-transfection of pmirGLO-BC069792-mut (miR-658 binding site mutation) and miR-658 mimics (MDA-MB-231: P = 0.253; MDA-MB-468: P = 0.152). After co-transfection of pmirGLO-BC069792-mut (miR-4739 binding site mutation) and miR-4739 mimics, there was no significant difference in luciferase activity between the experimental group and the control group (MDA-MB-231: P = 0.194; MDA- MB-468: P = 0.084). f There was no significant difference in luciferase activity between the experimental group and the control group after co-transfection of pmirGLO-KCNQ4-3'UTR-mut (miR-658 binding site mutation) and miR-658 mimics (MDA-MB-231: P = 0.160; MDA-MB-468: P = 0.699). After co-transfection of pmirGLO-KCNQ4-3'UTR-mut (miR-4739 binding site mutation) and miR-4739 mimics, there was no significant difference in luciferase activity between the experimental group and the control group (MDA-MB-231:P = 0.210; MDA-MB-468: P = 0.229).g-j In axillary tumors and lung metastases of mice, the expression of miR-658 and miR-4739 in LV-BC069792 group was lower than that in LV-NC group