Table 1 HIF-1-a levels under normoxia and in response to hypoxia in EwS cells
From: Hypoxia and HIFs in Ewing sarcoma: new perspectives on a multi-facetted relationship
Cell line | HIF-1-a detectable with western blot in normoxia | HIF-1-a detectable with western blot in hypoxia | Method to generate hypoxia | Reference |
|---|---|---|---|---|
SK-N-MC | No | Yes | 1%O2/ 5%CO2/ 95% N2 Spheroid growth | Aryee et al. 2010 |
Yes | - | 1%O2 | Hameiri Grossmann et al. 2015 | |
Yes, very low expression | Yes, strong expression | 0.1%O2/ 5%CO2/ balanceN2 | Knowles et al. 2010 | |
WE-68 | No | Yes | 1%O2/ 5%CO2/ 95% N2 Spheroid growth | Aryee et al. 2010 |
TC-252 | No Yes | Yes | 1%O2/ 5%CO2/ 95% N2 Spheroid growth | Aryee et al. 2010 |
TC-71 | Yes | Yes | 1%O2/ 5%CO2/ 95% N2 Spheroid growth | Aryee et al. 2010 |
No | Yes | 0.1%O2/ 5%CO2/ balanceN2 | Knowles et al. 2010 | |
RDES-1 | Yes | - | 1%O2 | Hameiri Grossmann et al. 2015 |
Yes, very low expression | Yes, strong expression | 0.1%O2/ 5%CO2/ balanceN2 | Knowles et al. 2010 | |
MHH-ES-1 | Yes | - | 1%O2 | Hameiri Grossmann et al. 2015 |
SK-ES-1 | Yes | Yes, levels unchanged | 1%O2 | Hameiri Grossmann et al. 2015 |
No | Yes | 0.1%O2 | Tilan et al. 2013 | |
No | Yes | 1%O2/ 5%CO2/ 95% N2 | Kling et al. 2020 | |
Yes, very low expression | Yes, strong expression | 0.1%O2/ 5%CO2/ balanceN2 | Knowles et al. 2010 | |
A-673 | No | Yes | 0.5%O2 | Kilic-Eren et al. 2013 |
No | Yes | 0.5%O2/ 5%CO2/ 95%N2 | Kilic et al. 2007 | |
No | Yes | 1%O2/ 5%CO2/ 95%N2 | Kling et al. 2020 | |
No | Yes | 0.1%O2/ 5%CO2 /balanceN2 | Knowles et al. 2010 | |
TC-32 | No | Yes | 1%O2 | El Naggar et al. 2015 |
CHLA-10 | Yes, very low expression | Yes, strong expression | 1%O2 | El Naggar et al. 2015 |
No | Yes | 1%O2/ 5%CO2/ 95%N2 | El Naggar et al. 2019 |