Fig. 4

EP300 knockdown inhibits circCCAR1 expression. A The H3K27ac signals in the CCAR1 promoter region in HepG2 cells were analyzed using ENCODE. B The H3K27ac signals in the CCAR1 promoter were measured using ChIP‒qPCR assay in LO2, HCCLM3 and SK-Hep-1 cells. ***p < 0.001 vs. LO2. C-D CCAR1 mRNA and circCCAR1 expression were measured by qRT‒PCR in HCC cells treated with C646 (10 μM) or DMSO for 48 h. **p < 0.01, ***p < 0.001 vs. DMSO. E Correlation analysis between EP300 and CCAR1 in TCGA liver hepatocellular carcinoma samples. F-G CCAR1 mRNA and circCCAR1 expression were detected after EP300 depletion. ***p < 0.001 vs. sh-NC. H EP300 and CCAR1 protein levels in HCC cells after EP300 depletion. I The EP300 signals in the CCAR1 promoter were measured using ChIP‒qPCR assays in LO2, HCCLM3 and SK-Hep-1 cells. ***p < 0.001 vs. LO2. J-K The H3K27ac signals in the CCAR1 promoter were measured using ChIP‒qPCR assays in HCC cells with EP300 silencing. ***p < 0.001 vs. sh-NC