Fig. 1

AZD4573 shows preclinical efficacy in DLBCL. A Cells were treated with 30 nM AZD4573 as indicated. Whole cell lysates were subjected to immunoblotting. Values for phosphorylated RNAPII are expressed numerically below the blots as a ratio of phosphorylated pRNAPII to total RNAPII, while RNAPII, MYC and MCL1 are expressed as a ratio of protein to Actin. B-C Proliferation was assessed in 9 DLBCL cell lines using a colorimetric tetrazolium-based assay, following 48-h treatment. Data is shown as mean ± SEM of three independent experiments, and a table of calculated IC50 values. IC50 was calculated using GraphPad Prism 9 software set to variable slope (four parameters). D Apoptosis was tested in 7 DLBCL cell lines treated with AZD4573, measured by flow cytometry at 24 h using Annexin-V staining. Data is shown as mean ± SEM of three independent experiments. *p < 0.05 and **p < 0.01 vs. untreated control