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Fig. 2 | Molecular Cancer

Fig. 2

From: Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma

Fig. 2

The expression and prognostic value of TOX2 in NKTL. A Expression (lg2) level of TOX2 in a collection of normal NK cells, NKTL cell line and NKTL patient samples derived from a microarray dataset in Gene Expression Omnibus (GEO) database (accession number: GSE80632). B Volcano plot demonstrating gene expression level in a collection of normal NK cells and NKTL patient samples derived from an RNA-seq dataset deposited in the Sequence Read Archive (SRA) database, under the accession code SRA200820. Y-axis represents p value (lg10). X-axis indicates the fold change (lg2) of genes differentially expressed between normal NK cells (left) and NKTL patient samples (right). TOX2 was labelled. C Quantitative RT-PCR of TOX2 gene expression in 3 normal NK cell samples and NKTL cell line NKYS, NK-92, HANK and NK-S1 (upper panel). The expressions of TOX2 gene were normalized to GAPDH level (internal control) for each sample and are presented as relative fold changes (n = 3, mean ± SD). *p < 0.01 for comparison of NKTL cell lines vs. normal NK cells. Western blotting analysis of TOX2 protein in one normal NK cell sample and 4 NKTL cell lines (lower panel). β-actin was used as a loading control. This result is representative for three independent biological replicates. D Utilizing data (GSE90784) from GEO database, TOX2 expression was categorized into TOX2- High (≥ 50%) and TOX2-Low (< 50%) group. Kaplan–Meier survival curves were constructed for NKTL patients based on TOX2 expression levels (TOX2-Low vs TOX2-High). Significance (p) was evaluated by Log-rank test. HR: hazard ratio

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