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Fig. 1 | Molecular Cancer

Fig. 1

From: Epigenetic reprogramming of Runx3 reinforces CD8 + T-cell function and improves the clinical response to immunotherapy

Fig. 1

DAC can trigger large-scale apparent reprogramming of CD8+ T cells. a Workflow of the experimental design including clinical sample collection and sequencing. b Schematic chart showing that DNA methylation reprogramming is correlated with clinical response and relapse. c Correlation analysis of tumor size and the anti-PD-1/DAC treatment cycles of patients. The patients were treated as indicated. Tumor size increase>50% were considered to indicate progression. d Analysis of genome-wide methylation variations in CD8 + T cells between the two indicated groups. The methylation was screened according to a |Diff beta value >0.1 and P< 0.05. Blue represents hypomethylation sites, and red represents hypermethylation sites. e Violin diagram showing the genome-wide methylation distribution of each patient. Red represents the monotherapy group, and blue represents the combined therapy group. The left panel is the baseline period of C1D0, and the right panel is the end of C2D0 treatment. f Statistical analysis of genome-wide DNA methylation levels. Upper panel: mean value of DNA methylation in the C1D0 and C2D0 periods; lower panel: median value of DNA methylation in the C1D0 and C2D0 periods (two-tailed unpaired t tests. n.s.: not significant, ***P < 0.001, ****P < 0.0001) g Distribution analysis of DNA methylation levels after anti-PD-1 or anti-PD-1/DAC. The DNA methylation value was divided into 20 sections from 0-1. A value < 0.15 was taken as the low methylation level, and a value > 0.85 was taken as the high methylation level. Upper panel: treated with anti-PD-1. Lower panel: treated with anti-PD1/DAC (left panel: before treatment; right panel: after treatment). h IPA pathway enrichment analysis. The input data are DMSs in each period. The size of the circle shows the number of enriched genes in each pathway, and the color depth represents the degree of enrichment

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