Fig. 4
The epigenetic sensitization effect of immunotherapy was eliminated in Runx3fl/fl;Lck-Cre mice. a Workflow of the construction of Runx3 conditional knockout mice and analysis of the tumor growth curve in Runx3fl/fl and Runx3fl/fl;Lck-Cre mice treated with anti-PD-1(n=5, two-tailed unpaired t tests, **P< 0.01). Upper panel: average tumor growth curves; lower panel: individual tumor growth curves. b Analysis of the tumor growth curve in Runx3fl/fl and Runx3fl/fl;Lck-Cre mice treated with anti-PD-1or DAC+ anti-PD-1(n=5,two-tailed unpaired t tests, **P<0.01). Upper panel: average tumor growth curves; lower panel: individual tumor growth curves. c tSNE analysis of immune cell subsets in the CD8+Tils of Runx3fl/fl and Runx3fl/fl;Lck-Cre mice treated with anti-PD-1. Upper panel: tSNE data showing the overall distribution of each subgroup. Lower panel: Histogram showing the absolute numbers of cells of various subtypes (cell number/104 CD45+ cells). d tSNE analysis of immune cell subsets in the CD8+ Tils of Runx3fl/fl and Runx3fl/fl;Lck-Cre mice treated with DAC+anti- PD-1. Upper panel: tSNE data showing the overall distribution of each subgroup. Lower panel: Histogram showing the absolute numbers of cells of various subtypes (cell number/104 CD45+ cells)