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Fig. 4 | Molecular Cancer

Fig. 4

From: CircZBTB44 promotes renal carcinoma progression by stabilizing HK3 mRNA structure

Fig. 4

HNRNPC enhanced the interaction of circZBTB44 and IGF2BP3 and mediated circZBTB44 translocation to cytoplasm via m6A modification. (A) The silencing efficiency of HNRNPC and IGF2BP3 in RCC cells was analyzed by qRT-PCR. (B) MeRIP assays were conducted to evaluate the impact of HNRNPC or IGF2BP3 knockdown on circZBTB44 m6A modification. (C) The m6A modification site of circZBTB44 was predicted on the SRAMP database (http://www.cuilab.cn/sramp). (D) RNA-pulldown assays were conducted to explore the interaction between HNRNPC and circZBTB44 or mutant circZBTB44. (E) qRT-PCR was used to detect the distribution of circZBTB44 in cytoplasm and nucleus of HNRNPC-silenced RCC cells. (F) RNA-pulldown assays were applied to investigate the effects of HNRNPC silencing on the binding between circZBTB44 and IGF2BP3 in RCC cells. (G) RIP assays were used to evaluate the impact of HNRNPC knockdown on the interaction of circZBTB44 and IGF2BP3 in RCC cells. ***P < 0.001

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