Fig. 5
From: CircZBTB44 promotes renal carcinoma progression by stabilizing HK3 mRNA structure
CircZBTB44 interacted with IGF2BP3 to regulate HK3 mRNA stability. (A) The catRAPID database (http://service.tartaglialab.com/page/catrapid_group) was used to predict the mRNAs that bound with IGF2BP3. (B) The impact of circZBTB44 knockdown on HK3 expression in RCC cells was examined using qRT-PCR analysis. (C) The enrichment of IGF2BP3 in the complex of HK3 3’UTR was analyzed using RNA pull-down assays. (D) RIP assays were used to verify the interaction of HK3 3’UTR and IGF2BP3 protein in RCC cells. (E) The effects of IGF2BP3 silencing on the mRNA stability of HK3 were examined using qRT-PCR in RCC cells treated with α-amanitin. (F) FISH assays were conducted to observe the colocalization of circZBTB44, IGF2BP3 and HK3 in RCC cells. (G) RIP assays were conducted to evaluate the impact of circZBTB44 knockdown on the interaction of HK3 3’UTR and IGF2BP3 in RCC cells. (H) qRT-PCR analysis was used to detect the expression efficiency of circZBTB44 in RCC cells. (I) qRT-PCR analysis was used to detect the HK3 expression in RCC cells after indicated transfection. **P < 0.01, ***P < 0.001