Fig. 8

CircZBTB44 promoted M2 polarization of macrophages by up-regulating HK3. (A) The co-culture system of the M0 macrophages with RCC cells. (B) Flow cytometry was applied to examine the ratio of CD86⁺CD206⁻ macrophages and CD206⁺CD86⁻ macrophages. (C) qRT-PCR and (D) Western blot were performed to evaluate the mRNA and protein expression of M1-assocaited genes (CD86, TNF-α) and M2-associated genes (CD206, ARG-1) in macrophages co-cultured with transfected RCC cells. *P < 0.05, **P < 0.01, ***P < 0.001