Fig. 2
From: Deciphering and advancing CAR T-cell therapy with single-cell sequencing technologies
Single-cell sequencing technologies for CAR T-cell therapy. (A) Schematic overview of single-cell multi-omics analysis of CAR T-cell therapy. (B) scRNA-seq measures the transcriptome from the entire cell. (C) scTCR-seq measures the V(D)J sequence of TCR and gene expression profiles in tandem. (D) scATAC-seq identifies open regions of chromatin by inserting adapters with Tn5 transposase to map regions of transcription factor binding. (E) CyTOF analyzes a high-dimensional, multi-parametric quantification of protein detection with metal-isotype antibody, inductively coupled plasma ionization, and time-of-flight detector. (F) CITE-seq allows the simultaneous assessment of the transcriptome and surface or intracellular protein expression of individual cells immunostained with oligonucleotide-coupled antibodies. (G) SCBC measures multiple secreted proteins with arrays of microchambers that are decorated with ordered arrays of antibodies against target proteins