Fig. 2From: The phospholipid transporter PITPNC1 links KRAS to MYC to prevent autophagy in lung and pancreatic cancerPITPNC1 inhibition in LUAD and PDAC cells reduce cell proliferation and impair tumour growth in vivo. A Western blot of PITPNC1 expression in A549, H358, H2009, H1792 LUAD cell lines and PATU8902, Panc1, MiaPaca2 PDAC cell lines transfected with a control (GFPsh) or a specific shRNA against PITPNC1 (PITPNC1 sh6 and sh7). Twenty μg of protein were loaded per sample. β-TUBULIN was used as loading marker. B Relative proliferation of A549 H23, H358, H2009, H1792, H2347 LUAD cell lines and PATU8902, Panc1, MiaPaca2 and HPAFII PDAC cell lines. Cells were transfected with a control (GFPsh) or a specific shRNA against PITPNC1 (PITPNC1 sh6 and sh7) (Dunnett´s multiple comparation test). C Representative images and quantification of clonogenic ability (mean ± std. error). D Tumour volume (mm3) of A549-derived xenografts (n = 6) (Dunnett’s multiple comparison test). E Representative images of tumours of D. F Tumour weight (g) of A549-derived xenografts (n = 6) of D at end point. G Tumour volume (mm3) of PATU8902-derived xenografts (n = 8) (Dunnett’s multiple comparison test). H Representative images of tumours of G. I Tumour weight (g) of PATU8902-derived xenografts (n = 8) of G at end point. J pH3 and CC3 quantification of A549-derived xenografts of D at end point. (Mann Whitney test). K pH3 and CC3 quantification of PATU8902-derived xenografts of G at end point (Mann Whitney test). L Representative images of lung photon flux ratio of A549 GFP/luciferase PITPNC1-overexpressing cells OE compared with the control (GFP/luciferase) (n = 8) at the indicated days. M Lung photon flux ratio of L (Bonferroni´s multiple comparison test). N Lung tumour nodules quantification on the lungs extracted from L (Mann Whitney test). O Liver foci quantification in the liver extracted from L (Mann Whitney test). P Representative images of lung tumour nodules quantification from N. Q Representative images of liver foci quantification from OBack to article page