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Fig. 2 | Molecular Cancer

Fig. 2

From: The phospholipid transporter PITPNC1 links KRAS to MYC to prevent autophagy in lung and pancreatic cancer

Fig. 2

PITPNC1 inhibition in LUAD and PDAC cells reduce cell proliferation and impair tumour growth in vivo. A Western blot of PITPNC1 expression in A549, H358, H2009, H1792 LUAD cell lines and PATU8902, Panc1, MiaPaca2 PDAC cell lines transfected with a control (GFPsh) or a specific shRNA against PITPNC1 (PITPNC1 sh6 and sh7). Twenty μg of protein were loaded per sample. β-TUBULIN was used as loading marker. B Relative proliferation of A549 H23, H358, H2009, H1792, H2347 LUAD cell lines and PATU8902, Panc1, MiaPaca2 and HPAFII PDAC cell lines. Cells were transfected with a control (GFPsh) or a specific shRNA against PITPNC1 (PITPNC1 sh6 and sh7) (Dunnett´s multiple comparation test). C Representative images and quantification of clonogenic ability (mean ± std. error). D Tumour volume (mm3) of A549-derived xenografts (n = 6) (Dunnett’s multiple comparison test). E Representative images of tumours of D. F Tumour weight (g) of A549-derived xenografts (n = 6) of D at end point. G Tumour volume (mm3) of PATU8902-derived xenografts (n = 8) (Dunnett’s multiple comparison test). H Representative images of tumours of G. I Tumour weight (g) of PATU8902-derived xenografts (n = 8) of G at end point. J pH3 and CC3 quantification of A549-derived xenografts of D at end point. (Mann Whitney test). K pH3 and CC3 quantification of PATU8902-derived xenografts of G at end point (Mann Whitney test). L Representative images of lung photon flux ratio of A549 GFP/luciferase PITPNC1-overexpressing cells OE compared with the control (GFP/luciferase) (n = 8) at the indicated days. M Lung photon flux ratio of L (Bonferroni´s multiple comparison test). N Lung tumour nodules quantification on the lungs extracted from L (Mann Whitney test). O Liver foci quantification in the liver extracted from L (Mann Whitney test). P Representative images of lung tumour nodules quantification from N. Q Representative images of liver foci quantification from O

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