Articles

A cluster of genes located in 1p36 are down-regulated in neuroblastomas with poor prognosis, but not due to CpG island methylation

A common feature of neuroblastoma tumours are partial deletions of the short arm of chromosome 1 (1p-deletions). This is indicative of a neuroblastoma tumour suppressor gene being located in the region. Severa...

Authors: Helena Carén, Katarina Ejeskär, Susanne Fransson, Luke Hesson, Farida Latif, Rose-Marie Sjöberg, Cecilia Krona and Tommy Martinsson

Global gene expression in neuroendocrine tumors from patients with the MEN1 syndrome

Multiple Endocrine Neoplasia type 1 (MEN1, OMIM 131100) is an autosomal dominant disorder characterized by endocrine tumors of the parathyroids, pancreatic islets and pituitary. The disease is caused by the fu...

Authors: William G Dilley, Somasundaram Kalyanaraman, Sulekha Verma, J Perren Cobb, Jason M Laramie and Terry C Lairmore

The loss of NKX3.1 expression in testicular – and prostate – cancers is not caused by promoter hypermethylation

Recent studies have demonstrated that the NKX3.1 protein is commonly down-regulated in testicular germ cell tumors (TGCTs) and prostate carcinomas. The homeobox gene NKX3.1 maps to chromosome band 8p21, which is ...

Authors: Guro E Lind, Rolf I Skotheim, Mario F Fraga, Vera M Abeler, Rui Henrique, Fahri Saatcioglu, Manel Esteller, Manuel R Teixeira and Ragnhild A Lothe

Insulin-like growth factor binding protein 2 promotes ovarian cancer cell invasion

Insulin-like growth factor binding protein 2 (IGFBP2) is overexpressed in ovarian malignant tissues and in the serum and cystic fluid of ovarian cancer patients, suggesting an important role of IGFBP2 in the b...

Authors: Eun-Ju Lee, Cristian Mircean, Ilya Shmulevich, Huamin Wang, Jinsong Liu, Antti Niemistö, John J Kavanagh, Je-Ho Lee and Wei Zhang

Evaluation of genome-wide chromatin library of Stat5 binding sites in human breast cancer

There is considerable interest in identifying target genes and chromatin binding sites for transcription factors in a genome-wide manner. Such information may become useful in diagnosis and treatment of diseas...

Authors: Matthew J LeBaron, Jianwu Xie and Hallgeir Rui

Identification of amplified and highly expressed genes in amplicons of the T-cell line huT78 detected by cDNA microarray CGH

Conventional Comparative Genomic Hybridization (CGH) has been widely used for detecting copy number alterations in cancer and for identifying regions containing candidate tumor responsible genes. Recently, sev...

Authors: Bárbara Meléndez, Beatriz Martínez-Delgado, Marta Cuadros, Victoria Fernández, Ramón Díaz-Uriarte and Javier Benítez

Gene expression profiling of tumours derived from ras^V12/E1A-transformed mouse embryonic fibroblasts to identify genes required for tumour development

In cancer, cellular transformation is followed by tumour development. Knowledge on the mechanisms of transformation, involving activation of proto-oncogenes and inactivation of tumour-suppressor genes has cons...

Authors: Sophie Vasseur, Cédric Malicet, Ezequiel L Calvo, Jean Charles Dagorn and Juan L Iovanna

Ectopic expression of PTTG1/securin promotes tumorigenesis in human embryonic kidney cells

Pituitary tumor transforming gene1 (PTTG1) is a novel oncogene that is expressed in most tumors. It encodes a protein that is primarily involved in the regulation of sister chromatid separation during cell div...

Authors: Tariq Hamid, Mohammed T Malik and Sham S Kakar

Stable expression of constitutively-activated STAT3 in benign prostatic epithelial cells changes their phenotype to that resembling malignant cells

Signal transducers and activators of transcription (STATs) are involved in growth regulation of cells. They are usually activated by phosphorylation at specific tyrosine residues. In neoplastic cells, constitu...

Authors: Hosea F Huang, Thomas F Murphy, Ping Shu, Arnold B Barton and Beverly E Barton

Dietary exposure to soy or whey proteins alters colonic global gene expression profiles during rat colon tumorigenesis

We previously reported that lifetime consumption of soy proteins or whey proteins reduced the incidence of azoxymethane (AOM)-induced colon tumors in rats. To obtain insights into these effects, global gene ex...

Authors: Rijin Xiao, Thomas M Badger and Frank A Simmen

Hypoxia-mediated apoptosis in oral carcinoma cells occurs via two independent pathways

We are attempting to elucidate the mechanism of apoptotic cell death induced by hypoxia in oral cancer cells. Since hypoxia can render solid tumors more resistant to radiation and chemotherapy, understanding t...

Authors: Nagathihalli S Nagaraj, Nadarajah Vigneswaran and Wolfgang Zacharias

Identification of a three-gene expression signature of poor-prognosis breast carcinoma

The clinical course of breast cancer is difficult to predict on the basis of established clinical and pathological prognostic criteria. Given the genetic complexity of breast carcinomas, it is not surprising t...

Authors: Ivan Bièche, Sengül Tozlu, Igor Girault and Rosette Lidereau

Dexamethasone protected human glioblastoma U87MG cells from temozolomide induced apoptosis by maintaining Bax:Bcl-2 ratio and preventing proteolytic activities

Glioblastoma is the deadliest and most prevalent brain tumor. Dexamethasone (DXM) is a commonly used steroid for treating glioblastoma patients for alleviation of vasogenic edema and pain prior to treatment wi...

Authors: Arabinda Das, Naren L Banik, Sunil J Patel and Swapan K Ray

Can gene expression profiling predict survival for patients with squamous cell carcinoma of the lung?

Lung cancer remains to be the leading cause of cancer death worldwide. Patients with similar lung cancer may experience quite different clinical outcomes. Reliable molecular prognostic markers are needed to ch...

Authors: Zhifu Sun, Ping Yang, Marie-Christine Aubry, Farhad Kosari, Chiaki Endo, Julian Molina and George Vasmatzis

Expression of the Tpl2/Cot oncogene in human T-cell neoplasias

Tpl2/Cot oncogene has been identified in murine T-cell lymphomas as a target of MoMuLV insertion. Animal and tissue culture studies have shown that Tpl2/Cot is involved in interleukin-2 (IL-2) and tumor necros...

Authors: Anna V Christoforidou, Helen A Papadaki, Andrew N Margioris, George D Eliopoulos and Christos Tsatsanis

CpG methylation of the FHIT, FANCF, cyclin-D2, BRCA2 and RUNX3 genes in Granulosa cell tumors (GCTs) of ovarian origin

Granulosa cell tumors (GCTs) are relatively rare and are subtypes of the sex-cord stromal neoplasms. Methylation induced silencing in the promoters of genes such as tumor suppressor genes, DNA repair genes and...

Authors: Varinderpal S Dhillon, Mohd Shahid and Syed Akhtar Husain

Mitochondrial inhibition of uracil-DNA glycosylase is not mutagenic

Uracil DNA glycosylase (UDG) plays a major role in repair of uracil formed due to deamination of cytosine. UDG in human cells is present in both the nucleus and mitochondrial compartments. Although, UDG's role...

Authors: Sushant Kachhap and Keshav K Singh

Saposin C promotes survival and prevents apoptosis via PI3K/Akt-dependent pathway in prostate cancer cells

In addition to androgens, growth factors are also implicated in the development and neoplastic growth of the prostate gland. Prosaposin is a potent neurotrophic molecule. Homozygous inactivation of prosaposin in ...

Authors: Tae-Jin Lee, Oliver Sartor, Ronald B Luftig and Shahriar Koochekpour

Is cumulative frequency of mitochondrial DNA variants a biomarker for colorectal tumor progression?

To examine the relationship between mitochondrial DNA (mtDNA) alterations and colorectal tumorigenesis, we used high-resolution restriction endonucleases and sequencing to assess the mitochondrial genome from ...

Authors: Felix O Aikhionbare, Masood Khan, Delicia Carey, Joel Okoli and Rodney Go

Activation of the hedgehog pathway in advanced prostate cancer

The hedgehog pathway plays a critical role in the development of prostate. However, the role of the hedgehog pathway in prostate cancer is not clear. Prostate cancer is the second most prevalent cause of cance...

Authors: Tao Sheng, Chengxin Li, Xiaoli Zhang, Sumin Chi, Nonggao He, Kai Chen, Frank McCormick, Zoran Gatalica and Jingwu Xie

A CpG island hypermethylation profile of primary colorectal carcinomas and colon cancer cell lines

Tumor cell lines are commonly used as experimental tools in cancer research, but their relevance for the in vivo situation is debated. In a series of 11 microsatellite stable (MSS) and 9 microsatellite unstable (...

Authors: Guro E Lind, Lin Thorstensen, Tone Løvig, Gunn I Meling, Richard Hamelin, Torleiv O Rognum, Manel Esteller and Ragnhild A Lothe

Gene expression profiling of epithelial ovarian tumours correlated with malignant potential

Epithelial ovarian tumours exhibit a range of malignant potential, presenting distinct clinical phenotypes. Improved knowledge of gene expression changes and functional pathways associated with these clinical ...

Authors: Susanne Warrenfeltz, Stephen Pavlik, Susmita Datta, Eileen T Kraemer, Benedict Benigno and John F McDonald

Molecular regulation of pancreatic stellate cell function

Until now, no specific therapies are available to inhibit pancreatic fibrosis, a constant pathological feature of chronic pancreatitis and pancreatic cancer. One major reason is the incomplete knowledge of the...

Authors: Robert Jaster

Genetic alterations and in vivo tumorigenicity of neurospheres derived from an adult glioblastoma

Pediatric brain tumors may originate from cells endowed with neural stem/precursor cell properties, growing in vitro as neurospheres. We have found that these cells can also be present in adult brain tumors an...

Authors: Patrizia Tunici, Lorena Bissola, Elena Lualdi, Bianca Pollo, Laura Cajola, Giovanni Broggi, Gabriella Sozzi and Gaetano Finocchiaro

RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells

Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. In order to understand how different cell types influence one another's gene exp...

Authors: Luiz OF Penalva, Michael D Burdick, Simon M Lin, Hedwig Sutterluety and Jack D Keene

Open Access gains attention in scholar communication

Authors: Paul J Chiao and Christian Schmidt

Aberrant CBFA2T3B gene promoter methylation in breast tumors

The CBFA2T3 locus located on the human chromosome region 16q24.3 is frequently deleted in breast tumors. CBFA2T3 gene expression levels are aberrant in breast tumor cell lines and the CBFA2T3B isoform is a pot...

Authors: Anthony J Bais, Alison E Gardner, Olivia LD McKenzie, David F Callen, Grant R Sutherland and Gabriel Kremmidiotis

Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity

Colorectal cancers displaying high-degree microsatellite instability (MSI-H) have an improved prognosis compared to microsatellite stable (MSS) cancers. The observation of pronounced lymphocytic infiltrates su...

Authors: Ayan Banerjea, Shafi Ahmed, Rebecca E Hands, Fei Huang, Xia Han, Peter M Shaw, Roger Feakins, Stephen A Bustin and Sina Dorudi

Molecular profiling of malignant peripheral nerve sheath tumors associated with neurofibromatosis type 1, based on large-scale real-time RT-PCR

Neurofibromatosis type 1 (NF1) is an autosomal dominant disorder with a complex range of clinical symptoms. The hallmark of NF1 is the onset of heterogeneous (dermal or plexiform) benign neurofibromas. Plexiform ...

Authors: Pascale Lévy, Dominique Vidaud, Karen Leroy, Ingrid Laurendeau, Janine Wechsler, Giulia Bolasco, Béatrice Parfait, Pierre Wolkenstein, Michel Vidaud and Ivan Bièche

Mutation in mitochondrial complex I ND6 subunit is associated with defective response to hypoxia in human glioma cells

Hypoxia-tolerant human glioma cells reduce oxygen consumption rate in response to oxygen deficit, a defense mechanism that contributes to survival under moderately hypoxic conditions. In contrast, hypoxia-sens...

Authors: Carrie DeHaan, Bahram Habibi-Nazhad, Elizabeth Yan, Nicole Salloum, Matthew Parliament and Joan Allalunis-Turner

PTTG/securin activates expression of p53 and modulates its function

Pituitary tumor transforming gene (PTTG) is a novel oncogene that is expressed abundantly in most tumors. Overexpression of PTTG induces cellular transformation and promotes tumor formation in nude mice. PTTG has...

Authors: Tariq Hamid and Sham S Kakar

Liver-specific expression of the agouti gene in transgenic mice promotes liver carcinogenesis in the absence of obesity and diabetes

The agouti protein is a paracrine factor that is normally present in the skin of many species of mammals. Agouti regulates the switch between black and yellow hair pigmentation by signalling through the melano...

Authors: Alexander I Kuklin, Randall L Mynatt, Mitchell L Klebig, Laura L Kiefer, William O Wilkison, Richard P Woychik and Edward J Michaud

Role of promoter hypermethylation in Cisplatin treatment response of male germ cell tumors

Male germ cell tumor (GCT) is a highly curable malignancy, which exhibits exquisite sensitivity to cisplatin treatment. The genetic pathway(s) that determine the chemotherapy sensitivity in GCT remain largely ...

Authors: Sanjay Koul, James M McKiernan, Gopeshwar Narayan, Jane Houldsworth, Jennifer Bacik, Deborah L Dobrzynski, Adel M Assaad, Mahesh Mansukhani, Victor E Reuter, George J Bosl, Raju SK Chaganti and Vundavalli VVS Murty

Phospholipase C δ-4 overexpression upregulates ErbB1/2 expression, Erk signaling pathway, and proliferation in MCF-7 cells

The expression of the rodent phosphoinositide-specific phospholipase C δ-4 (PLCδ4) has been found to be elevated upon mitogenic stimulation and expression analysis have linked the upregulation of PLCδ4 express...

Authors: David W Leung, Chris Tompkins, Jim Brewer, Alexey Ball, Mike Coon, Valerie Morris, David Waggoner and Jack W Singer

CHK2 kinase expression is down-regulated due to promoter methylation in non-small cell lung cancer

CHK2 kinase is a tumor suppressor that plays important role in DNA damage signaling, cell cycle regulation and DNA damage induced apoptosis. CHK2 kinase expression was known to be ubiquitous in mammalian cells...

Authors: Peilin Zhang, Jie Wang, Weiyi Gao, Bao-Zhu Yuan, John Rogers and Eddie Reed

Epigenetic CRBP downregulation appears to be an evolutionarily conserved (human and mouse) and oncogene-specific phenomenon in breast cancer

The cellular retinol binding protein I gene (CRBP) is downregulated in a subset of human breast cancers and in MMTV-Myc induced mouse mammary tumors. Functional studies suggest that CRBP downregulation contribute...

Authors: Alice Arapshian, Silvina Bertran, Yuvarani S Kuppumbatti, Shigeo Nakajo and Rafael Mira-y-Lopez

L1 and HERV-W retrotransposons are hypomethylated in human ovarian carcinomas

Wide-spread hypomethylation of CpG dinucleotides is characteristic of many cancers. Retrotransposons have been identified as potential targets of hypomethylation during cellular transformation. We report the r...

Authors: Laura Menendez, Benedict B Benigno and John F McDonald

Comparison of gene expression in HCT116 treatment derivatives generated by two different 5-fluorouracil exposure protocols

Established colorectal cancer cell lines subjected to different 5-fluorouracil (5-FU) treatment protocols are often used as in vitro model systems for investigations of downstream cellular responses to 5-FU and t...

Authors: Paula M De Angelis, Katherine L Kravik, Siv H Tunheim, Terje Haug and Wenche H Reichelt

Scambio, a novel guanine nucleotide exchange factor for Rho

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades and mitogenesis. For this reason, a major objectiv...

Authors: Christina Curtis, Bianca Hemmeryckx, Leena Haataja, Dinithi Senadheera, John Groffen and Nora Heisterkamp

Genetic alteration and gene expression modulation during cancer progression

Cancer progresses through a series of histopathological stages. Progression is thought to be driven by the accumulation of genetic alterations and consequently gene expression pattern changes. The identificati...

Authors: Cathie Garnis, Timon PH Buys and Wan L Lam

The proteosome inhibitor MG132 attenuates Retinoic Acid Receptor trans-activation and enhances trans-repression of Nuclear Factor κB. Potential relevance to chemo-preventive interventions with retinoids

Nuclear factor kappa B (NFκB) is a pro-malignant transcription factor with reciprocal effects on pro-metastatic and anti-metastatic gene expression. Interestingly, NFκB blockade results in the reciprocal induc...

Authors: Valentine B Andela and Randy N Rosier

Regulation of COX-2 protein expression by Akt in endometrial cancer cells is mediated through NF-κB/IκB pathway

Cyclooxygenase-2 (COX-2) has been shown to be highly expressed in a broad series of primary endometrial tumors and its expression may be closely associated with parameters of tumor aggressiveness. In human end...

Authors: Marie-Eve St-Germain, Veronique Gagnon, Sophie Parent and Eric Asselin

Genome characteristics of primary carcinomas, local recurrences, carcinomatoses, and liver metastases from colorectal cancer patients

Colorectal cancer (CRC) is one of the most common causes of cancer-related deaths in the Western world, and despite the fact that metastases are usually the ultimate cause of deaths, the knowledge of the genet...

Authors: Chieu B Diep, Manuel R Teixeira, Lin Thorstensen, Johan N Wiig, Mette Eknæs, Jahn M Nesland, Karl-Erik Giercksky, Bertil Johansson and Ragnhild A Lothe

Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR

Probe based detection assays form the mainstay of transcript quantification. Problems with these assays include varying hybridization efficiencies of the probes used for transcript quantification and the expen...

Authors: Vincenzo Pagliarulo, Ben George, Stephen J Beil, Susan Groshen, Peter W Laird, Jie Cai, James Willey, Richard J Cote and Ram H Datar

Expression of the pro-angiogenic factors vascular endothelial growth factor and interleukin-8/CXCL8 by human breast carcinomas is responsive to nutrient deprivation and endoplasmic reticulum stress

The expression of pro-angiogenic cytokines, such as vascular endothelial growth factor (VEGF) and interleukin-8/CXCL8 (IL-8), plays an important role in tumor growth and metastasis. Low oxygen tension within p...

Authors: Philip L Marjon, Ekaterina V Bobrovnikova-Marjon and Steve F Abcouwer

N-Methyl-N'-nitro-N-nitrosoguanidine-induced senescence-like growth arrest in colon cancer cells is associated with loss of adenomatous polyposis coli protein, microtubule organization, and telomeric DNA

Cellular senescence is a state in which mammalian cells enter into an irreversible growth arrest and altered biological functions. The senescence response in mammalian cells can be elicited by DNA-damaging age...

Authors: Aruna S Jaiswal, Asha S Multani, Sen Pathak and Satya Narayan

Retraction Note: TGFβ₁ signaling via a_Vβ₆ integrin

Authors: Martin P Kracklauer, Christian Schmidt and Guido M Sclabas

Retraction Note: TGFβ₁ activates c-Jun and Erk1 via a_Vβ₆ integrin

Authors: Karsta Luettich and Christian Schmidt

Regulation of COX-2 transcription in a colon cancer cell line by Pontin52/TIP49a

Cyclooxygenase-2 (COX-2) is expressed early in colon carcinogenesis and is known to play a crucial role in the progress of the disease. Here we show that the regulation of the expression of this enzyme in a co...

Authors: Mary L Carlson, Ellen T Wilson and Stephen M Prescott

Role of APC and DNA mismatch repair genes in the development of colorectal cancers

Colorectal cancer is the third most common cause of cancer-related death in both men and women in the western hemisphere. According to the American Cancer Society, an estimated 105,500 new cases of colon cance...

Authors: Satya Narayan and Deodutta Roy