Figure 5
Analysis of SP and non-SP used for cell isolation and for animal experiments. A) RC-58T/hTERT/SA#4-D cells were subjected to Hoechst 33342 dye and separated into SPlow, SPmid, and SPhi fractions B). Individual SP fractions were then injected subcutaneously and assayed for tumorigenicity, after 100 days in NOD-SCID mice. Tumors were subsequently measured for tumor volume as shown by schematic representing xenograft tumors. D). Formalin-fixed, paraffin-embedded tissues were obtained from SP generated xenograft tumors. Individual tissues sections were stained with H&E or human specific primary PSCA antibody, and Alexa Fluor-594 secondary (red) and Dapi (blue) nuclear stain. Scale bar indicates 40 μM.