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Figure 3 | Molecular Cancer

Figure 3

From: Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) is a potential tumour suppressor in prostate cancer and is frequently silenced by promoter methylation

Figure 3

UCHL1 expression in wild type and stably transfected prostate cancer cell lines. UCHL1 mRNA (upper panel) and protein (lower panel) are abundantly expressed in DU145, while LNCaP cells show an absence of UCHL1 expression. The housekeeping gene RPLP0 for RT-PCR and GAPDH for Western blotting confirm equivalent loading of samples. Exogenous stable expression of UCHL1 is obtained in androgen dependant prostate cancer cells (LNCaP) by retroviral transduction. For confirmation of expression of UCHL1 mRNA and protein, RT-PCR (upper panel) and Western blotting (lower panel) were performed respectively.

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Molecular Cancer

ISSN: 1476-4598