Figure 4
From: Anti-tumor effects of fibroblast growth factor-binding protein (FGF-BP) knockdown in colon carcinoma
Anti-proliferative effects of FGF-BP knockdown are based on alterations in phospho-MAPK status. Phospho-MAPK Proteome Profiler antibody array probed with lysates from FGF-BP knockdown (grey bars) vs. mock-transfected (black bars) LS174T cells (A). Original blots with Akt1, Akt2 and GSK3β signals being highlighted by red frames (B, left); observed differences are statistically significant (B, center). Results from antibody arrays are confirmed in independent experiments by Western blotting for Akt (B, upper right) and p21 (B, lower right; see Figure 5 for array).