Figure 3
From: Effects of 1α,25 dihydroxyvitamin D3 and testosterone on miRNA and mRNA expression in LNCaP cells
Validation of changes in the mRNA levels of AR, VDR, PSA, TMPRSS2 and CYP24A1. LNCaP cells were treated with 5 nM T and 100 nM 1,25(OH)2D3 alone and in combination and transcript levels were measured by Real Time-PCR over a 72 h time course. Fold changes greater than 0.8 are statistically significant; values within the shaded areas in each graph are not significantly modulated (0.8 or below after transformation). Comparisons between different treatment groups were analyzed using one-way ANOVA; differences were considered significant if p < 0.05 (*), NS: not significant. Significant changes (p < 0.05) in at least two out of three time points were required for the changes to be considered biologically relevant. Note: Scales on the ordinate axis vary from transcript to transcript.