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Figure 2 | Molecular Cancer

Figure 2

From: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex

Figure 2

GABPα and β subunit protein and mRNA levels are decreased in the SK-BR-3 cell line. (a) Western blot analysis of whole cell lysates from MCF-7, T-47D and SK-BR-3 cells was carried out using antibodies to GABPα, GABPβ and the blots were then reprobed with anti-γ-tubulin as an internal control. Apparent molecular weight markers (kDa) are indicated to the right of the panels. (b) The relative transcript levels of the GABP subunits in MCF-7 (M), T-47D (T) and SK-BR-3 (S) cells were examined by semi-quantitative RT-PCR. Specific products were amplified from equal amounts of RT product from the cell lines indicated using primer sets for GABPα, GABPβ and GAPDH as an internal control. Products were separated on a 1.5% agarose gel with 100 bp ladder in leftmost lane. (c) Quantitative RT-PCR analysis of GABP beta-41 subunit mRNA was carried out on the indicated cell lines. Levels are expressed in relation to the 184hTERT cell line.

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Molecular Cancer

ISSN: 1476-4598