Skip to main content
Figure 7 | Molecular Cancer

Figure 7

From: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex

Figure 7

NRF-1 binds to the GABPβ promoter. (a) An oligonucleotide with a known NRF-1 binding site (RC4) [22] was used as a probe in an EMSA with MCF-7 nuclear extracts and Gb-270 as a cold competitor. NRF-1 binding (NRF-1), non-specific (NS) binding and free probe (F) are indicated. (b) Recombinant NRF-1 (rNRF-1) was prepared as a fusion with the maltose binding protein. Decreasing amounts of recombinant protein (5, 2.6, 1.3, 0.26, 0.13 and 0.03 μg) were used in an EMSA with RC4 and Gb-270 probes. Binding complexes as indicated above. (c) A ChIP assay was performed using MCF-7 chromatin and antibodies against acetylated histone H3K9 (acH3), haemagglutinin (HA, negative control), NRF-1 and Oct-4 (transcription factor, negative control). PCR products obtained using primers specific to the GABPβ promoter (refer to Methods) are shown.

Back to article page

Molecular Cancer

ISSN: 1476-4598