Concordant and opposite roles of DNA-PK and the

Table 1 Proteins identified by LC-MS/MS of cut bands in the Ku86 complex

Protein name¹	Synonym¹	Accession Number¹	Theoretical MW (kDa)¹	No. of Peptides²	Function³
ATP-dependent DNA helicase 2 subunit 1	Ku70	P12956	69.71	21	DNA repair
ATP-dependent DNA helicase 2 subunit 2	Ku86	P13010	82.57	20	DNA repair
FACT complex subunit SPT16⁴	SPT16	Q9Y5B9	119.91	11	Nucleosome/transcription
Werner syndrome ATP-dependent helicase	WRN	Q14191	162.49	7	Helicase
Lamina-associated polypeptide 2 isoform alpha	LAP2A	P42166	75.36	7	DNA binding
DNA topoisomerase 1	TOP1	P11387	90.73	1	Chromatin binding
Poly (ADP-ribose) polymerase 1	PARP-1	P09874	112.95	1	DNA binding
ATP-dependent RNA helicase	DDX17	Q92841	72.37	1	Helicase

¹ as in the SWISS-PROT database, ² number of peptide obtain by LC-MSMS analysis of the unique silver stained band shown in figure 1, ³ as in the Gene Ontology classification.

ISSN: 1476-4598