Figure 5

Unlike periostin, the carboxy-terminus of rTGFBI supports adhesion of ovarian cancer cells and is dependent on an intact RGD motif. A, Coomassie brilliant blue stained SDS-PAGE of full-length and various truncated constructs of rTGFBI purified from bacteria. B, SKOV3 cells replated on tissue culture wells either uncoated or coated with poly-L-lysine, fibronectin and various rTGFBI constructs for 30 minutes. Bright-field images (a-f) were processed following Giemsa staining. rTGFBI comprises aa 1–683, fourth FasI comprises aa 497–637, C-terminus comprises aa 497–683, and central domain comprises aa 24–506. Scale bar = 400 μm. C, Adhesion results of three independent experiments were normalized to poly-L-lysine and represented as percent of fibronectin control. Significance of ***p < 0.001 when comparing fourth FasI, central domain, and C-terminus RGDmut to full-length TGFBI. D, Coomassie stained SDS-PAGE of purified recombinant full-length and C-terminus of periostin along with C-terminus of TGFBI. E, SKOV3 cells were replated on tissue culture wells coated with indicated constructs and allowed to adhere for 30 minutes. Results are of three independent experiments, normalized to poly-L-lysine, and represented as percent of fibronectin control, *p < 0.05, ***p < 0.001.