Table 2 The list of sequences of primers used for real-time RT-PCR experiments
Primers | Sequences |
|---|---|
Scramblase | Forward: 5'-AATGATTGGTGCCTGTTTCC -3' Reverse: 5'-TCCACTACCACACTCCTGATTT -3' |
Floppase | Forward: 5'-TTGAACTAGGCAGCATCAGC-3' Reverse: 5'-GAACAGTGTCAACAGGCCAAT-3' |
Argonaute | Forward: 5’-TTCATCGTGGTGCAGAAGAG-3’ Reverse: 5’-CCCAGAGGTATGGCTTCCTT-3’ |
Dicer | Forward: 5’-TCGCTGGCTGTAAAGTACGA-3’ Reverse: 5’-TTCAAGCAATTCTCGCACAG-3’ |
Drosha | Forward: 5’-TGCAACTGGTAGCCACAGAG-3’ Reverse: 5’-ACACTGCTGAAGCTGGGATT-3’ |
GAPDH | Forward: : 5’-TGCCAAATATGATGACATCAAGAA-3’ Reverse: 5’-GGAGTGGGTGTCGCTGTTG-3’ |
miR-U6 | Forward: 5’-CTCGCTTCGGCAGCACA-3’ Reverse: 5’-AACGCTTCACGAATTTGCGT-3’ |
miR-107 | 5'-AGCAGCATTGTACAGGGCTATC-3' |
miR-125b | 5'-TCCCTGAGACCCTAACTTGTGA-3' |
miR-150 | 5'-TCTTCCCAACCCTTGTACCAGTG-3' |
miR-210 | 5'-CTGTGCGTGTGACAGCGGCTGA-3' |