Figure 2
From: Asymmetric segregation of template DNA strands in basal-like human breast cancer cell lines
Frequency of asymmetric segregation of template DNA and correlation with Her2 expression. (A) Percentages of cells with asymmetric division of BrdU-labeled DNA templates are shown for 12 breast cancer cell lines. Analyses were performed in triplicates in triplicates and at least two independent experiments were performed. Basal cell lines had a higher frequency of ACD as compared to luminal cell lines (P = 0.03, Fisher’s exact test) (B) The breast cancer cell lines were divided into two groups according to the percentage of BrdU asymmetric division observed, high asymmetry (mean, ≥ 0.5%, which was the limit of quantifiable detection) and low asymmetry (mean, <0.5%). Relative Her2 expression, as assessed by flow cytometry in at least two independent experiments, is shown as mean fluorescence intensity. Cell lines with high asymmetry had lower Her2 expression as compared to cell lines with low asymmetry (P = 0.02, Mann–Whitney test). Error bars are standard error of the mean.