Figure 4
From: Regulation of PMP22 mRNA by G3BP1 affects cell proliferation in breast cancer cells
G3BP1 increases cell proliferation. MCF-7 cells were transiently transfected with expression vectors encoding FLAG-tagged G3BP1, G3BP2a or G3BP2b for 24 hours (A, B) prior to [3H]-thymidine incubation for 6 hours or qPCR analysis. Western blot confirms G3BP overexpression (C). Data (mean ± SEM, n = 3) are expressed as CPM relative to control. qPCR data (mean ± SEM, n = 3) of PMP22 expression were normalized to three house-keeping genes SDHA, YWHAZ and UBC and related to control sample. * < 0.05, according to ANOVA followed by Duncan’s multiple range test.