Figure 4

β-catenin expression in untreated, TQ-low, TQ-high, or piroxicam treated APC^Minmice (n=8). Membranous (A), cytoplasmic (B), and nuclear (C) β-catenin IRSs were calculated for epithelial cells of the normal mucosa, small and large polyps. Bar graphs display mean β-catenin IRSs ± SD. TQ-high treated mice (E) showed an increase in membranous β-catenin expression in large polyps (ANOVA, Dunnett, 2-sided, *p<0.05) and a trend for an increase in small polyps (p=0.064) when compared to untreated (D) APC^Min mice. Membranous β-catenin expression in piroxicam treated mice did not reach significance (p=0.094) due to a too small number of large polyps, although a membranous relocalization of the protein was present as in the TQ-high treated mice. Cytoplasmic expression of β-catenin increased upon TQ-low and TQ-high (p=0.064) treatment in the normal mucosa. Magnification: 40x (left panel) and 400x (right panel). Panel F shows the negative control sample without the primary antibody (F).