Variants | Primer sequence (5′ to 3′) | PCR products | Anneal T | Allele | ||
---|---|---|---|---|---|---|
Upper-forward; lower-reverse | (°C) | Enzymes used | determination | |||
NF-α-308G > A (rs1800629) | AGGCAATAGCTTTTGAGGGCCATG | 80 bp | 60 | Sty I | Intact | :-308GG |
TCTTCTGGGCCACTGACTGAT | ||||||
58 bp + 22 bp | :-308GA + AA | |||||
TNF-α-857C > T (rs1799724) | CGAGTATGGGGACCCTCCCATTAA | 100 bp | 57 | Ase1 | Intact | :-857CC |
ACGTCCCCTGTATTCCATACC | ||||||
78 bp + 22 bp | :-857CT + TT | |||||
TNF-α-863C > A (rs1800630) | GACCACAGCAATGGGTAGGAGA | 86 bp | 60 | Bsl I | Intact | :-863CC |
TCTACATGGCCCTGTCCTCGT | ||||||
64 bp + 22 bp | :-863CA + AA | |||||
TNF-α-1031T > C (rs1799964) | AGAGCTGTGGGGAGAACAAA | 247 bp | 62 | Bbs I | Intact | :-1031TT |
CTCCTACCCATTGCTGTGGT | ||||||
127 bp + 120 bp | :-1031TC + CC |