Figure 3

Regulation of MAP7 expression by miR-16 at both the transcript and protein levels. (A, D and G) Relative quantification RT-PCR analysis of miR-16 levels in A549 (A), Hela (D) and MCF-7 (G) cells treated with pre-miR-16, pre-miR-control, anti-miR-16 or anti-miR-control. The results presented are the mean ± SD of three independent experiments (*** p < 0.001). (B, E and H) Relative quantification RT-PCR analysis of MAP7 mRNA levels in A549 (B), Hela (E) and MCF-7 (H) cells treated with pre-miR-16, pre-miR-control, anti-miR-16 or anti-miR-control. The results presented are the mean ± SD of three independent experiments (*** p < 0.001). (C, F and I) Western blot analysis of MAP7 protein levels in A549 (C), Hela (F) and MCF-7 (I) cells treated with pre-miR-16, pre-miR-control, anti-miR-16 or anti-miR-control. Left panel: representative image; right panel: quantitative analysis (*** p < 0.001).