Figure 6
From: Small nucleolar RNA signatures of lung tumor-initiating cells
Effect of snoRA42 suppression on tumor formation of CD133+ cells isolated from NSCLC cell lines and expressions of stem cell-associated genes. (A) After six weeks, 5 X 105 Calu-1-derived CD133+ cells transfected with scrambled siRNA were able to generate tumor xenografts (red arrow) in all six mice. However, 5 X 105 Calu-1-derived CD133+ cells transfected with snoRA42-siRNA could not produce tumor in any mice. (B) Knockdown of snoRA42 using snoRA42-siRNA in H-1944- and Calu-1-derived CD133+ cells resulted in a significant reduction in transcriptional activity of stem-associated genes determined by qRT-PCR.