Figure 6

DEPP overexpression leads to a sensitization to etoposide-induced apoptosis. a) Real-time PCR of SH-EP cells set under serum starvation (0.5% FCS) for 24 hours and treated with 20 μg/ml etoposide for 6 hours. Shown are means ± s.e.m. of three independent experiments; statistical analysis was done with the Student’s unpaired t-test, ***P < 0.01. b) SH-EP/tetEGFP and SH-EP/tetDEPP cells were treated with 20 μg/ml etoposide alone and in combination with 200 ng/ml doxy for 48 hours. PI-FACS analyses were performed to detect apoptotic cells. Shown are mean values ± s.e.m. of three independent experiments; statistical analysis was done with the Student’s unpaired t-test, *P < 0.05, **P < 0.025.