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Figure 3 | Molecular Cancer

Figure 3

From: F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation

Figure 3

FBXL19 targets Rac3 for ubiquitination - A. HEK293 cells were co-transfected with Flag tagged Rac3 (Rac3-Flag) and FBXL19-V5 plasmids. Cell lysates were subjected to immunoprecipitation with a Flag tag antibody, followed by V5 tag immunoblotting. Input lysates were analyzed by immunoblotting with V5 tag and Flag tag antibodies. B. HEK293 cells were transfected with FBXL19-V5 plasmid followed by MG-132 (20 μM) treatment. Cell lysates were subjected to immunoprecipitation with a V5 tag antibody, followed by Rac3 immunoblotting. Input lysates were analyzed by immunoblotting with Rac3 and β-actin antibodies. C. MLE12 cells were co-transfected with Rac3-V5 and FBXL19-HA plasmids. Cell lysates were subjected to immunoprecipitation with an ubiquitin antibody, followed by V5 tag immunoblotting. Input lysates were analyzed by immunoblotting with HA tag, V5 tag and β-actin antibodies. All the blots are representative of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598