Figure 2

The direct regulation of VEGF by RBP2 in human gastric cancer cells. QRT-PCR analyses of (A) RBP2 and (B) VEGF mRNA level in control and RBP2 siRNA (10 nM)-transfected BGC-823, SGC-7901 and GES-1 cell lines after 72 h. **P < 0.01. Western blot analyses of (C, D) RBP2 and VEGF protein levels and (E, F) tri- and di- methylation of H3K4 in cancer cells treated with control and RBP2 siRNAs. C and R: Control and RBP2 siRNA, respectively. **P < 0.01. QRT-PCR analyses of (G) RBP2 and (H) VEGF mRNA levels in cancer cells transfected with control and RBP2 expression vector (4 μg) after 48 h. **P < 0.01. (I, J) Western blot analyses of RBP2 and VEGF protein levels and (K, L) tri- and di- methylation of H3K4 in cancer cells treated with control and RBP2 expression vectors. Vector C and R: Control pcDNA3.1 and pcDNA3.1-RBP2 expression plasmids, respectively. **P < 0.01. Data are mean ± SEM of 3 independent experiments.