Table 2 Correlation of promoter methylation with clinical and histologic parametrs, HPV status and microsatellite instability in CC patients

Parameter

Overall

CDH1

DAPK

RARB

HIC1

FHIT

RASSF1A

APC

CDKN2A

MGMT

BRCA1

Histology

   SCC (N = 77)

66

45

36

24

12

9

5

5

6

4

3

   AC (N = 5)

5

0

1

0

3

0

1

4

1

0

2

Significance

P = 1.00

P = 0.02

P = 0.372

P = 0.315

P = 0.040

P = 1.00

P = 0.323

P = 0.0003

P = 0.367

P = 1.00

P = 0.028

Stage

   I (N = 17)

10

6

5

3

5

0

4

2

2

0

0

   II (N = 19)

19

13

12

5

3

3

2

2

1

0

2

   III-IV (N = 46)

42

16

20

16

7

6

0

5

4

4

3

Significance

P = 0.0005

P = 0.0005

P = 0.129

P = 0.394

P = 0.460

P = 0.279

P = 0.003

P = 1.00

P = 0.759

P = 0.395

P = 0.397

Size of tumor

   1–5 cm (N = 32)

28

20

13

10

8

3

4

4

1

0

1

   ≥ 6 cm (N = 38)

34

21

19

13

6

4

1

3

4

2

2

Significance

P = 1.000

P = 0.540

P = 0.433

P = 0.793

P = 0.337

P = 1.00

P = 0.171

P = 0.695

P = 0.366

P = 0.497

P = 1.000

Age (yrs)

   ≤ 40 (N = 32)

28

17

17

12

6

6

1

1

3

3

1

   41–50 (N = 17)

12

8

5

5

3

0

2

0

1

1

1

   51–60 (N = 13)

13

6

5

6

3

2

1

6

1

0

3

   > 60 (N = 20)

18

14

10

1

3

1

2

2

2

0

0

Significance

P = 0.113

P = 0.439

P = 0.400

P = 0.021

P = 0.956

P = 0.169

P = 0.600

P = 0.0004

P = 1.000

P = 0.467

P = 0.053

Clinical Outcome

Alive (N= 39)

30

18

19

8

7

3

5

4

2

0

0

DOC (N= 43)

41

27

18

16

8

6

1

5

5

4

5

Significance

0.015

P = 0.131

P = 0.533

P = 0.097

P = 0.939

P = 0.487

P = 0.097

P = 1.000

P = 0.436

P = 0.118

P = 0.056

Treatment response¶

CR (N= 21)

19

11

15

3

4

1

2

3

2

0

0

DOC/PR (N = 26)

24

15

10

12

4

3

1

4

3

2

2

Significance

1.000

0.716

0.024

0.020

1.000

0.617

0.579

1.000

1.000

0.495

0.495

HPV type

   HPV 16 (N = 47)

42

27

24

15

9

6

4

5

3

3

2

   HPV 18 (N = 6)

5

2

1

1

2

0

1

1

1

0

1

   Multiple (N = 11)

8

7

3

2

1

0

0

2

2

0

1

   Others (N = 16)

12

9

8

5

2

3

0

0

1

1

1

significance

P = 0.543

P = 0.740

P = 0.280

P = 0.792

P = 0.560

P = 0.467

P = 0.365

P = 0.288

P = 0.327

P = 1.000

P = 0.412

MSI

   MSS (N = 30)

26

20

17

4

2

3

1

2

5

4

2

   MSI-L (N = 9)

9

6

4

2

1

2

1

0

1

0

2

   MSI-H (N = 9)

8

2

3

3

4

0

1

3

1

0

1

Significance

P = 0.516

P = 0.065

P = 0.513

P = 0.434

P = 0.023

P = 1.000

P = 0.313

P = 0.079

P = 1.000

P = 0.443

P = 0.266