Figure 3
Enhanced Tyrosine Phosphorylation of proteins associated with the integrin-cytoskeleton-complex. Cytoskeletally anchored αVβ6 was immunoprecipitated after TGFβ1 and/or fibronectin stimulation (10 nM for 10 minutes) followed by Western analysis with antibodies against tyrosine-phosphorylated proteins (A). Reprobing with αV and β6 antibodies show equal anounts of precipitates used (B).