Proteins that cooperate with Ets1 | |||
---|---|---|---|
protein | responsive gene(s) | comments | reference |
AML-1 | TCRα, TCRβ, osteopontin, GM-CSF | mutual blockage of the negative regulatory domains induces cooperative binding of Ets1 and AML-1 | |
ATF-2 | TCRα | binds stronger to Ets1 when it lacks dimerization domain | [48] |
AP-1 (c-Jun/c-Fos) | GM-CSF, TIMP-1, polyoma enhancer, MMP-1 | Â | |
CBP/p300 | MMP-3 | Â | |
ERK1/2 | uPA, MMP-3, prolactin | phosphorylates threonine-38 | |
Ets1 | MMP-3 | requires presence of a palindromic sequence | [61] |
Estrogen receptor | artificial promoter | Ets1 cooperates with unliganded receptor | [62] |
GATA3 | human interleukin-5 | requires presence of PMA and ionomycin | [63] |
HIF-2α | VEGFR-2 (Flk-1) |  | [64] |
HTLV-I Tax | PTHrP (P3 promoter), interleukin-5 | Tax forms ternary complex with Ets1 and Sp1, enhances GATA3/Ets1 cooperation | |
LEF-1 | TCRα | LEF-1 induces DNA-bending that facilitates Ets1/ATF2 interaction | [48] |
c-Myb | α4 integrin, mim-1, lck type I | Ets1/c-Myb synergism blocks repression by ZEB | |
NFAT | HIV-LTR | Â | [69] |
NF-κB | GM-CSF, HIV-LTR |  | |
Pax5 | mb-1 | allows Ets1 to bind to unfavorable GGAG, changes contacts of Y395 (H3-helix) with DNA | |
Pit-1/GHF-1 | prolactin | Â | [73] |
PKCα | PTHrP (P3 promoter) | constitutively active form increases Ets1 activity and induces phosphorylation of exon VII domain | [74] |
mutant p53 | MDR1 | wildtype p53 does not bind to and synergize with Ets1 | [75] |
wildtype p53 | mdm2, bax | requires presence of CBP, and UV-radiation, Ets1 necessary for p53-dependent apoptosis in ES cells | [76] |
Ras | uPA, MMP-3, prolactin | induces ERK1/2-dependent phosphorylation of threonine-38 | |
Smad3/4 | PTHrP (P3 promoter) | requires presence of TGFβ | [77] |
SPBP | synthetic promoter | SPBP = stromelysin-1 PDGF responsive element binding protein | [78] |
Sp1 | PTHrP, MRG1, HTLV-I LTR, integrin αIIb, P4 promoter of MVMp, FasL | Ets1/Sp1 bind cooperatively to DNA | |
Sp100 | MMP3, synthetic promoters | does not activate ΔVII-Ets1 | [45] |
Stat5 | GAS/Ets elements | Â | [84] |
TFE3 | immunoglobulin μ heavy-chain gene | basic helix-loop-helix domain of TFE3 sufficient for Ets1 binding, several Ets1 domains involved in binding | [46] |
huUBC9 | synthetic promoter | Â | [85] |
USF-1 | HIV-LTR | Â | [86] |
Vitamin D receptor (VDR) | artificial promoter | Ets1 induces conformational change of VDR, in the presence of Ets1 activation by VDR does not require AF2-domain | [62] |
Proteins that repress Ets1 activity | |||
protein | responsive gene | comments | reference |
CaMKII | GM-CSF | blocks AML-1/Ets1 cooperative effect by phosphorylating exon VII domain | [47] |
Daxx/EAPI (Ets1-associated protein) | MMP1 and BCL2 | a nuclear protein that cooperates with Ets1 to repress transcription | [87] |
EAPII (Ets1-associated protein) | MMP1 | a nuclear protein that attenuates Ets1/AP1 cooperative effect, inhibits migration of epithelial cells | [55] |
MafB (AP-1 likeprotein) | transferrin receptor, porphobilinogen deaminase | suppresses Ets1-dependent activation of erythoid-specific genes | [88] |
ZEB | α4 integrin | repression of Ets1 by ZEB is blocked when Ets1 synergizes with c-Myb | [67] |