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Figure 12 | Molecular Cancer

Figure 12

From: p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growth

Figure 12

TGFβ-1 increases p8 expression in Panc-1 cells. Panc-1 cells were treated with TGFβ-1 (5 ng/ml) for 12 hours under standard conditions. Total RNA and protein were prepared and p8 mRNA and p8 protein expressions were measured by RT-PCR and Western blot respectively as described in Material and Methods. RL3 mRNA expression was used as a control in RT-PCR experiments and β-tubulin as a housekeeping control protein in Western blots.

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