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Figure 13 | Molecular Cancer

Figure 13

From: p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growth

Figure 13

p8 transcription is activated by TGFβ-1 through Smad4 in Panc-1 cells. Panc-1 cells were cultivated in 30 mm diameter culture dishes for 24 hours and then transiently transfected with 0.5 μg of p8-CAT and 0.5 μg of pCMV/βgal plasmids. Plamids encoding constitutively activated type I TGFβ receptor (RI ACT), dominant negative type II TGFβ receptor (RII DN), dominant negative of Smad4 (DPC4 1–514 a.a.) and the Smad4 wild type (0.5 μg) were co-transfected with p8-CAT and pCMV/βgal plasmids in the presence or not of TGFβ-1 (5 ng/ml) or the p38 inhibitor SB203580 as indicated. Cell extracts were prepared 24 hours after transfection and CAT and β-galactosidase activities were measured. CAT activity was normalized to β-galactosidase activity. Experiments were carried out in triplicate and repeated two or three times.

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Molecular Cancer

ISSN: 1476-4598