Table 2 Oligonucleotide primers used for MSP
From: CHK2 kinase expression is down-regulated due to promoter methylation in non-small cell lung cancer
Wild Type forward | (-615) CCTCC C GGGCTCAAG C GATTCTCCT GCCT (-587) |
|---|---|
Methylated forward | (-615) TTTTT C GGGTTTAAG C GATTTTTTT GTTT (-587) |
Unmethylated forward: | (-615) TTTTT T GGGTTTAAG T GATTTTTTT GTTT (-587) |
Wild type reverse: | (-411) GCG CG GGGGT TCACTCCTGT AGTCCCG CA (-439) |
Methylated reverse: | (-411) ACG CG AAAAT TCACTCCTAT AATCCCG CA (-439) |
Unmethylated reverse: | (-411) ACA CA AAAAT TCACTCCTAT AATCCCA CA (-439) |