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Figure 3 | Molecular Cancer

Figure 3

From: Phospholipase C δ-4 overexpression upregulates ErbB1/2 expression, Erk signaling pathway, and proliferation in MCF-7 cells

Figure 3

PLC assays in PLCδ4 overexpressing cells and expression of GFP-PH domain/PLCδ4 fusion protein in MCF-7 cells. Assay of enzymatic activities of PLCδ4 overexpressing clones in MCF-7 cell extracts were carried out using PIP2 (panel A) or PI (panel B) as the substrate. EGFP refers to cells transfected with a control vector expressing EGFP (Clonetech, Palo Alto, CA); MCF-7 refers to untransfected cells; #1, 2, 4, 5, 10, and 11 refer to activities derived from total cell extracts of independent isolates of PLCδ4 overexpressing clones. #2 nuclei refers to activities derived from nuclear extracts of PLCδ4 overexpressing clone #2. Assays were done in triplicates with error bars indicating ± standard deviation (SD). Fusion protein comprised of the PH domain of PLCδ4 and GFP was localized mainly in the cytoplasmic region (panel C).

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Molecular Cancer

ISSN: 1476-4598