Figure 8
PLCδ4 overexpressing cells form colonies in soft agar. The cells were plated at 1 × 105 cells/ml in 6-well plates containing RPMI 1640 with 0.35% agar, 5% FBS, 10% tryptose phosphate broth, 100 nM 17-β-estradiol. The picture was taken on day 10 with 40× magnification. Samples were plated on duplicate wells. The two numbers separated by a slash mark shown at the lower right-hand corner of each panel represent the total number of colonies scored in each of the two wells.