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Figure 3 | Molecular Cancer

Figure 3

From: RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells

Figure 3

Reassortment of mRNA and PABP was not detected in cell lysates. The potential for displacement of PABP was tested by adding increasing amounts (0–1500 μg) of pure competitor poly (A) to 400 μl of cell lysates of PY4.1 and T98G cells prior to incubation with antibody-coated (anti-PABP) beads. Following immunoprecipitation, mRNAs were isolated from the pellets and analyzed using the RNase Protection Assay (RPA) mouse angiogenesis (mAngio) and human tumor suppressor (hTS1) multi-probe sets. Unprotected RNA probes were used to identify the nature of the different sized protected fragments. The experiment shows that the interaction between PABP and the endogenous mRNA targets cannot be disrupted by competing poly (A) RNA.

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Molecular Cancer

ISSN: 1476-4598